|
KMID : 0613820230330080612
|
|
Journal of Life Science
2023 Volume.33 No. 8 p.612 ~ p.622
|
|
|
Three-Dimensional Culture of Thymic Epithelial Cells Using Porous PCL/PLGAComposite Polymeric Scaffolds Coated with Polydopamine
|
|
Choi Seung-Mi
Lee Do-Young
Lim Ye-Seon
Hwang Seon-Yeong
Song Won-Hoon
Jeong Young-Hun
Yoon Sik
|
|
|
Abstract
|
|
|
|
T-cell deficiency may occur in various clinical conditions including congenital defects, cell/organ transplantation, HIV infection and aging. In this regard, the development of artificial thymus has recently been attracting much attention. To achieve this aim, the development of techniques for 3D culture of thymic stromal cells is necessary because thymocytes grown only in a 3D thymic microenvironment can be differentiated fully to become mature, immunocompetent T cells; the same cannot be achieved for thymocytes grown in 2D. This study aimed to develop a nanotechnology-based 3D culture technique using polymeric scaffolds for thymic epithelial cells (TECs), the main component of thymic stromal cells. Scanning electron microscopic observation revealed that the pores of both PCL and PCL/PLGA scaffolds were filled with TECs. Interestingly, TECs grown in 3D on polydopamine-coated scaffolds exhibited enhanced cell attachment and proliferation compared to those grown on non-coated scaffolds. In addition, the gene expression of thymopoietic factors was upregulated in TECs cultured in 3D on polydopamine-coated scaffolds compared to those cultured in 2D. Taken together, the results of the present study demonstrate an efficient 3D culture model for TECs using polymeric scaffolds and provide new insights into a novel platform technology that can be applied to develop functional, biocompatible scaffolds for the 3D culture of thymocytes. This will eventually shed light on techniques for the in vitro development of T cells as well as the synthesis of artificial thymus.
|
|
|
KEYWORD
|
|
|
3D cell culture, polycaprolactone (PCL), polydopamine, poly-lactic-co-glycolic acid (PLGA), thymic epithelial cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|